Ole Miss will have nighttime football right out of the gate in 2016.
The inaugural Orlando Kickoff will feature Ole Miss and Florida State with a 7 p.m. kickoff and ESPN television.
Ole Miss was No. 10, Florida State No. 14 in the final Associated Press rankings for 2015.
The Rebels had a morning kickoff and an afternoon kickoff in their first two games of last season.
The Orlando game will mark the second time in three years that Ole Miss will begin its season at a neutral site. The Rebels defeated Boise State 35-13 to open the 2014 season in Atlanta.
The school is also in talks with Baylor for a possible game in Houston in 2020.