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The Mississippi Highway Patrol tells WTVA TV Betty Turvaville died at the North Mississippi Medical Center where she was being treated following the accident.
Turvaville was the driver of a van that was hit by a dump truck at the intersection of Highway 30 Bypass and County Road 5031 around 7:45 a.m. Friday.
Thirty-five-year-old Becky A. Hatfield, a passenger in the van, died at the scene of the accident.
The dump truck driver was not injured.
The accident remains under investigation.