Senators originally defeated the bill Jan. 25, but it was brought back up for a second round of debate Wednesday. This time, it passed 29-18.
The bill moves to the House for more work.
The bill would allow the import and export of farm-raised white tail deer, its semen, ova, and embryos.
Senate Wildlife, Fisheries and Parks Committee Chairman Tommy Gollott of Biloxi says farmers also could enclose the deer in pens and sell hunts.
The Mississippi Wildlife Federation is among the groups opposing the farms.
The bill is Senate Bill 2530.