The reunion tour will feature all four of the band's original members, Bret Michaels, Rikki Rockett, Bobby Dall and C.C. DeVille.
Poison reached its peak in the 80s, selling more than 30 million records worldwide. Its biggest hit is the ballad "Every Rose Has Its Thorn."
The rock band will also release a two-disc greatest-hits collection on May 3 called "Double Dose: Ultimate Hits."
Warrant is opening the show.
Tickets go on sale Friday at 10 a.m. and are $99.50/VIP after party, $45 and $25, all plus fees, at the BancorpSouth Arena Box office, all Ticketmaster locations or charge by phone at (800) 745-3000 or (662) 841-6528.