The Daily Corinthian reports that Rhonda Greening announced her resignation on Tuesday in a letter to the chamber's board of directors.
Greening said she felt that after the loss of city funding to the chamber her salary would be too much of a strain on the organization's budget.
Greening had served as executive director of the chamber since 2006.
The city had been providing about $45,000 a year to the chamber of commerce.
Mayor Joe Eaton says the city will continue providing free office space and utilities.